NMR 400 CHECKLIST

You must be able to do these things before you will be allowed to run on your own.

1. Be able to check to see if someone is running, how to stop, save and remove their sample…how not to put a tube in if one is already in the magnet.

2. Clean, check for camber, inspect bottom and top, insert and eject tube….know where the emergency eject is, know not to leave your sample in depth gauge.

3. Open software, join experiments, switch experiments, create new experiments, find experiments.

4.  Lock with proper gain, power, phase, and field (Z0), know when to read in standard shim file….what to do when you can’t lock.

5.  How to read in and save shim files, how to shim manually on Z1C and Z2C, how to gradient shim, how to tell when it is bad, no gradient shimming on 2 shifts or more of deuterium, how to tell when you need to shim the non-spins and doing so.

6.  How to run a spectrum in GLIDE, how to change a parameter before running, like # of scans (nt).

7.  How to run more than one spectrum in GLIDE consecutively.

8.  Be able to run a 1D proton, a 1D carbon (understand the S/N check), a simple DEPT, a simple COSY.

9.  Know where to look things up in the manuals.

10.  Be able to work up a spectrum manually, including phase correction, integrating and setting standard integral, vp, baseline correction, referencing,  printing with variations.

11.  Be able to read spectra in and out of experiments, work on one while running another.

12.  Be able to move or copy files to Lylestation, backup on CD, use reservation software.

13.  Know how to get us for trouble!!!!

14. Where to go on our website for information on moving files, backing up files to CD, etc. Link: http://www.unh.edu/instrumentation-center/help-users.htm